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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence information will not be but out there.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually has the epitope regarded from the antibody.
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This guideline outlines even further achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This guidebook outlines further more achievable optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide now not bind into the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the really conserved cyclin relatives, whose members are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins more info purpose as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S section boundary and is also degraded as cells development by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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